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mutant reporter m51 super 89 fopflash  (Addgene inc)


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    Structured Review

    Addgene inc mutant reporter m51 super 89 fopflash
    Mutant Reporter M51 Super 89 Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+reporter+m51+super+89+fopflash/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm27350215-76-20-30
    Average 96 stars, based on 574 article reviews
    mutant reporter m51 super 89 fopflash - by Bioz Stars, 2026-09
    96/100 stars

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    Transfection:

    Article Title: C-mannosylation of R-spondin3 regulates its secretion and activity of Wnt/β-catenin signaling in cells.
    Article Snippet: .. After 24 h, cells were transiently transfected with 400 ng of canonical Wnt signaling reporter M50 Super 89 TOPFlash or mutant reporter M51 Super 89 FOPFlash (gifted from Randall Moon; Addgene plasmids #12456 and #12457) [19,29] and 5 ng of phRL-TK vector (Promega) (Fig. 3E), or with 800 ng of TOPFlash or FOPFlash and 20 ng of phRL-TK vector (Fig. 4D) in the presence of Wnt3a conditioned medium, produced from L-Wnt3a cells (ATCC, Manassas, VA, USA; CRL-2647) as previously reported [30]. .. After 24 h, cells were lysed and luciferase activities were measured using infinite 200Pro (Tecan Co., M€annedorf, Switzerland).

    Mutagenesis:

    Article Title: C-mannosylation of R-spondin3 regulates its secretion and activity of Wnt/β-catenin signaling in cells.
    Article Snippet: .. After 24 h, cells were transiently transfected with 400 ng of canonical Wnt signaling reporter M50 Super 89 TOPFlash or mutant reporter M51 Super 89 FOPFlash (gifted from Randall Moon; Addgene plasmids #12456 and #12457) [19,29] and 5 ng of phRL-TK vector (Promega) (Fig. 3E), or with 800 ng of TOPFlash or FOPFlash and 20 ng of phRL-TK vector (Fig. 4D) in the presence of Wnt3a conditioned medium, produced from L-Wnt3a cells (ATCC, Manassas, VA, USA; CRL-2647) as previously reported [30]. .. After 24 h, cells were lysed and luciferase activities were measured using infinite 200Pro (Tecan Co., M€annedorf, Switzerland).

    Plasmid Preparation:

    Article Title: C-mannosylation of R-spondin3 regulates its secretion and activity of Wnt/β-catenin signaling in cells.
    Article Snippet: .. After 24 h, cells were transiently transfected with 400 ng of canonical Wnt signaling reporter M50 Super 89 TOPFlash or mutant reporter M51 Super 89 FOPFlash (gifted from Randall Moon; Addgene plasmids #12456 and #12457) [19,29] and 5 ng of phRL-TK vector (Promega) (Fig. 3E), or with 800 ng of TOPFlash or FOPFlash and 20 ng of phRL-TK vector (Fig. 4D) in the presence of Wnt3a conditioned medium, produced from L-Wnt3a cells (ATCC, Manassas, VA, USA; CRL-2647) as previously reported [30]. .. After 24 h, cells were lysed and luciferase activities were measured using infinite 200Pro (Tecan Co., M€annedorf, Switzerland).

    Produced:

    Article Title: C-mannosylation of R-spondin3 regulates its secretion and activity of Wnt/β-catenin signaling in cells.
    Article Snippet: .. After 24 h, cells were transiently transfected with 400 ng of canonical Wnt signaling reporter M50 Super 89 TOPFlash or mutant reporter M51 Super 89 FOPFlash (gifted from Randall Moon; Addgene plasmids #12456 and #12457) [19,29] and 5 ng of phRL-TK vector (Promega) (Fig. 3E), or with 800 ng of TOPFlash or FOPFlash and 20 ng of phRL-TK vector (Fig. 4D) in the presence of Wnt3a conditioned medium, produced from L-Wnt3a cells (ATCC, Manassas, VA, USA; CRL-2647) as previously reported [30]. .. After 24 h, cells were lysed and luciferase activities were measured using infinite 200Pro (Tecan Co., M€annedorf, Switzerland).



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    mutant reporter m51 super 89 fopflash  (Addgene inc)
    96
    Addgene inc mutant reporter m51 super 89 fopflash
    Mutant Reporter M51 Super 89 Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutant+reporter+m51+super+89+fopflash/M50+Super+8x+TOPFlash+(Plasmid+%2312456)/pm27350215-76-20-30
    Average 96 stars, based on 1 article reviews
    mutant reporter m51 super 89 fopflash - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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